Coding
ATA-rtTA3

Part:BBa_K1179004:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-rtTA3) A membrane/exosome targeting rtTA3 fusion to send through exosomes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Keep in mind that the Acyl-TyA sequence exports the protein into exosomes so protein extraction and other quantification protocols are needed to test successful experimental function.

Source

The TyA protein is derived from a yeast retrotransposon virus-like particle and when added with the Acylation tag it acts as an exosome export protein, allowing the rtTA to be exported when fused to the TyA.

References